Application of third-generation sequencing in cancer research

Abstract In the past several years, nanopore sequencing technology from Oxford Nanopore Technologies (ONT) and single-molecule real-time (SMRT) Pacific BioSciences (PacBio) have become available to researchers are currently being tested for cancer research. These methods offer many advantages over most widely used high-throughput short-read approaches allow comprehensive analysis of transcriptomes by identifying full-length splice isoforms other posttranscriptional events. addition, these platforms enable structural variation characterization at a previously unparalleled resolution direct detection epigenetic marks in native DNA RNA. Here, we present summary important applications technologies research, including identification complex structure variants, alternatively spliced isoforms, fusion transcript events, exogenous Furthermore, discuss impact newly developed RNA (RNA-Seq) approach advancing epitranscriptome research cancer. Although unique challenges still new long-read methods, they will unravel aspects genome complexity unprecedented ways an encouraging outlook continued application increasing number different settings.